| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Drozdowski, Jessica L (13)
|
NITWST |
2:31.27 |
2:35.05 |
3.78 |
| 2 |
Lee, Ella (13)
|
NICLSC |
|
2:41.76 |
- |
| 3 |
Marshall, Olivia J (14)
|
NIEMAC |
2:40.78 |
2:43.91 |
3.13 |
| 4 |
Buell, Peyton E (13)
|
NIHAMB |
2:48.70 |
2:44.52 |
-4.18 |
| 5 |
Musterait, Madeline A (14)
|
NITWST |
2:43.74 |
2:44.97 |
1.23 |
| 6 |
Pecorella, Brooke E (13)
|
NIEMAC |
2:55.17 |
2:48.77 |
-6.40 |
| 7 |
Kubiak, Kelly A (13)
|
NITWST |
2:46.54 |
2:49.42 |
2.88 |
| 8 |
Sainsbury, Meghan A (13)
|
NITWST |
2:54.97 |
2:52.28 |
-2.69 |
| 9 |
Heinrich, Chloe M (14)
|
NITWST |
3:00.96 |
2:54.07 |
-6.89 |
| 10 |
Higley, Kayla P (14)
|
NIRAYS |
|
2:55.18 |
- |
| 11 |
Machowski, Stella J (13)
|
NITWST |
3:06.89 |
2:58.25 |
-8.64 |
| 12 |
Keesler, Kaylynn N (13)
|
NIEMAC |
2:51.92 |
3:00.06 |
8.14 |
| 13 |
Machowski, Erin M (13)
|
NIHAMB |
3:04.75 |
3:05.31 |
0.56 |
| 14 |
Gonzalez, Catherine E (13)
|
NIHAMB |
3:08.30 |
3:06.44 |
-1.86 |
| 15 |
Regan, Abby S (14)
|
NITWST |
3:14.48 |
3:12.57 |
-1.91 |
| 16 |
Hellwig, Ashleigh R (14)
|
NIHHSC |
3:44.18 |
3:36.77 |
-7.41 |
|
Flaherty, Katie T (14)
|
NIEAST |
2:55.90 |
NS |
- |